[Xplor-nih] Distance from PRE

[Marius Clore]

First, you shouldn¹t use a single time point measurement but a two-time
point measurement. The former is inaccurate.
See: Iwahara, J., Tang, C. & Clore, G.M. (2007) Practical aspects of 1H
transverse paramagnetic relaxation enhancement measurements on
macromolecules. J. Magn. Reson. 184, 185-195.

Second, you should be refining directly against the PRE rates. See: Iwahara,
J., Schwieters, C. D., Clore, G. M. (2004) Ensemble approach for NMR
structure refinement against 1H paramagnetic relaxation enhancement data
arising from a flexible paramagnetic group attached to a macromolecule. J.
Am. Chem. Soc. 126, 5879-5896.


On 4/7/08 10:37 AM, "Suja Naren" <sujanaren at yahoo.com> wrote:

> 
> Hi,
>      I like to estimate the distance from PRE single time point  measurement.
> Can anyone help me with this?
> 
> Thanks a lot,
> Suja
> 
>  
> 
> You rock. That's why Blockbuster's offering you one month of Blockbuster Total
> Access 
> <http://us.rd.yahoo.com/evt=47523/*http://tc.deals.yahoo.com/tc/blockbuster/te
> xt5.com> , No Cost.
> 
> _______________________________________________
> Xplor-nih mailing list
> Xplor-nih at nmr.cit.nih.gov
> http://dcb.cit.nih.gov/mailman/listinfo/xplor-nih


-- 
G. Marius Clore, MD, PhD
Chief, Protein NMR Section
Laboratory of Chemical Physics
Bldg 5/Rm B1-30I
NIDDK, National Institutes of Health
Bethesda, MD 20892-0520
Tel: (301) 496 0782
Fax: (301) 496 0825
E-mail: mariusc at intra.niddk.nih.gov
web: http://spin.niddk.nih.gov/clore


-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://dcb.cit.nih.gov/pipermail/xplor-nih/attachments/20080407/a4e43dcd/attachment.html